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验证数据展示

  • WB analysis using 31442-1-AP

    WB analysis using 31442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31442-1-AP (WASF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 31442-1-AP

    IHC staining of human placenta using 31442-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31442-1-AP (WASF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 31442-1-AP

    IF Staining of U2OS using 31442-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using WASF2 antibody (31442-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis of U2OS using 31442-1-AP

    WB analysis of U2OS using 31442-1-AP

    WB result of WASF2 antibody (31442-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WASF2 transfected U2OS cells.

  • Featured Product
  • KD/KO验证

WASF2 Polyclonal antibody

WASF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31442-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Protein WAVE 2, SCAR2, WAS protein family, member 2, WASP family protein member 2, WAVE2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, U2OS cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31442-1-AP targets WASF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 WASF2 fusion protein Ag35663 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAS protein family, member 2
别名 Protein WAVE 2, SCAR2, WAS protein family, member 2, WASP family protein member 2, WAVE2
计算分子量54kDa，498aa
观测分子量80 kDa
GenBank蛋白编号NM_006990.4
基因名称 WASF2
Gene ID (NCBI) 10163
RRIDAB_3669985
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y6W5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASF2 (WASp family Verprolin-homologous protein-2), also known as WAVE2, a member of the Wiskott-Aldrich syndrome protein (WASp) family of actin nucleation promoting factors, is a downstream effector molecule that implicates the signal transduction from small GTPases to the actin cytoskeleton, which can elongate the cell movement mode (PMID: 35849030,28332566). Moreover, WASF2 promoted the invasiveness of cancer cells by binding to actin cytoskeletal protein alpha-actinin 4 (ACTN4) (PMID: 30353690). The calculated MW of WASF2 is 54 kDa, with an isoform of 32 kDa, but the observed MW is about 80 kDa, which may be due to post-translational modification (PMID: 35849030,28332566,21383498,17389688,18362183,38391912).

实验方案

Product Specific Protocols
WB protocol for WASF2 antibody 31442-1-APDownload protocol
IHC protocol for WASF2 antibody 31442-1-APDownload protocol
IF protocol for WASF2 antibody 31442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols