验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy VPS53 Ready-To-Use IHC Kit. VPS53 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67610-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-67610 targets VPS53 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | VPS53 fusion protein Ag29875 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 53 homolog (S. cerevisiae) |
| 别名 | FLJ10979, HCCS1, hVps53L, pp13624, VPS53 |
| 计算分子量 | 832 aa, 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC029560 |
| 基因名称 | VPS53 |
| Gene ID (NCBI) | 55275 |
| RRID | AB_2934684 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q5VIR6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 VPS53 antibody CL555-67610 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |