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验证数据展示

  • WB analysis using 67610-1-Ig

    WB analysis using 67610-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human liver using 67610-1-Ig

    IHC staining of human liver using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VPS53 antibody included
  • IHC staining of human liver using 67610-1-Ig

    IHC staining of human liver using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VPS53 antibody included
  • IF Staining of HEK-293 using 67610-1-Ig

    IF Staining of HEK-293 using 67610-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67610-1-Ig (VPS53 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

VPS53 Monoclonal antibody

VPS53 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67610-1-Ig

产品说明书

CloneNo. 2B12C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

2B12C3, PP13624, Vacuolar protein sorting-associated protein 53 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, 4T1 cells
Positive IHC detected inhuman liver tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy VPS53 Ready-To-Use IHC Kit. VPS53 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67610-1-Ig targets VPS53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VPS53 fusion protein Ag29875 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 53 homolog (S. cerevisiae)
别名 2B12C3, PP13624, Vacuolar protein sorting-associated protein 53 homolog
计算分子量 832 aa, 94 kDa
观测分子量 94 kDa
GenBank蛋白编号BC029560
基因名称 VPS53
Gene ID (NCBI) 55275
RRIDAB_2882816
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ5VIR6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

实验方案

Product Specific Protocols
WB protocol for VPS53 antibody 67610-1-IgDownload protocol
IHC protocol for VPS53 antibody 67610-1-IgDownload protocol
IF protocol for VPS53 antibody 67610-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols