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验证数据展示

  • WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

    WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VPS53 antibody included
  • IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VPS53 antibody included
  • IF Staining of HEK-293 using 67610-1-Ig (same clone as 67610-1-PBS)

    IF Staining of HEK-293 using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67610-1-Ig (VPS53 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

VPS53 Monoclonal antibody, PBS Only

VPS53 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67610-1-PBS

产品说明书

CloneNo. 2B12C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2B12C3, PP13624, Vacuolar protein sorting-associated protein 53 homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VPS53 Ready-To-Use IHC Kit. VPS53 primary antibody included.

产品信息

67610-1-PBS targets VPS53 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29875

Product name: Recombinant human VPS53 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-128 aa of BC029560

Sequence: MVLLDLPSISSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPLVVFVDNYIKLLTDCNTETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLIKKRL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 53 homolog (S. cerevisiae)
别名 2B12C3, PP13624, Vacuolar protein sorting-associated protein 53 homolog
计算分子量 832 aa, 94 kDa
观测分子量 94 kDa
GenBank蛋白编号BC029560
基因名称 VPS53
Gene ID (NCBI) 55275
RRIDAB_2882816
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5VIR6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

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