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验证数据展示

  • WB analysis using 14272-1-AP (same clone as 14272-1-PBS)

    WB analysis using 14272-1-AP (same clone as 14272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

  • WB analysis of U-937 using 14272-1-AP (same clone as 14272-1-PBS)

    WB analysis of U-937 using 14272-1-AP (same clone as 14272-1-PBS)

    WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 14272-1-AP (same clone as 14272-1-PBS)

    IHC staining of human kidney using 14272-1-AP (same clone as 14272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VPS4A antibody included
  • Featured Product
  • KD/KO Validated

VPS4A Polyclonal antibody, PBS Only

VPS4A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14272-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EC:3.6.4.6, hVPS4, Protein SKD2, SKD1, SKD2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® VPS4A Ready-To-Use IHC Kit. VPS4A primary antibody included.

产品信息

14272-1-PBS targets VPS4A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5592

Product name: Recombinant human VPS4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 86-437 aa of BC047932

Sequence: KENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 4 homolog A (S. cerevisiae)
别名 EC:3.6.4.6, hVPS4, Protein SKD2, SKD1, SKD2
计算分子量 49 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC047932
基因名称 VPS4A
Gene ID (NCBI) 27183
RRIDAB_2878039
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UN37
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vacuolar Protein Sorting 4A (VPS4A) is one of two human homologs of the yeast protein Vps4 and member of the AAA protein family (ATPases associated with diverse cellular activities).

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