验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
14272-1-AP targets VPS4A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 | VPS4A fusion protein Ag5592 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 4 homolog A (S. cerevisiae) |
| 别名 | hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A |
| 计算分子量 | 49 kDa |
| 观测分子量 | 48-55 kDa |
| GenBank蛋白编号 | BC047932 |
| 基因名称 | VPS4A |
| Gene ID (NCBI) | 27183 |
| RRID | AB_2878039 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UN37 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS4A antibody 14272-1-AP | Download protocol |
| IHC protocol for VPS4A antibody 14272-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Parasitol Res Microautophagy upregulation in cutaneous lymph nodes of dogs naturally infected by Leishmania infantum. | ||
Theranostics Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy | ||
