验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
14272-1-AP targets VPS4A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 | VPS4A fusion protein Ag5592 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 4 homolog A (S. cerevisiae) |
| 别名 | EC:3.6.4.6, hVPS4, Protein SKD2, SKD1, SKD2 |
| 计算分子量 | 49 kDa |
| 观测分子量 | 48-55 kDa |
| GenBank蛋白编号 | BC047932 |
| 基因名称 | VPS4A |
| Gene ID (NCBI) | 27183 |
| RRID | AB_2878039 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UN37 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vacuolar Protein Sorting 4A (VPS4A) is one of two human homologs of the yeast protein Vps4 and member of the AAA protein family (ATPases associated with diverse cellular activities).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS4A antibody 14272-1-AP | Download protocol |
| IHC protocol for VPS4A antibody 14272-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Parasitol Res Microautophagy upregulation in cutaneous lymph nodes of dogs naturally infected by Leishmania infantum. | ||
Theranostics Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy | ||
