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VPS37A Monoclonal antibody

VPS37A Monoclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1F9A4

Cat No : 66327-1-Ig

Print datasheet

Synonyms

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

验证数据展示

  • WB analysis of SW 1990 using 66327-1-Ig

    WB analysis of SW 1990 using 66327-1-Ig

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66327-1-Ig

    IHC staining of human kidney using 66327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 66327-1-Ig

    IHC staining of human kidney using 66327-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MCF-7 using 66327-1-Ig

    IF Staining of MCF-7 using 66327-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inSW 1990 cells, MCF-7 cells
Positive IHC detected inhuman kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66327-1-Ig targets VPS37A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen VPS37A fusion protein Ag19108 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vacuolar protein sorting 37 homolog A (S. cerevisiae)
Synonyms ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC022363
Gene Symbol VPS37A
Gene ID (NCBI) 137492
RRIDAB_2881708
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NEZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

实验方案

Product Specific Protocols
WB protocol for VPS37A antibody 66327-1-IgDownload protocol
IHC protocol for VPS37A antibody 66327-1-IgDownload protocol
IF protocol for VPS37A antibody 66327-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Endocrinol (Lausanne)

Stratification of diabetic kidney diseases via data-independent acquisition proteomics–based analysis of human kidney tissue specimens

Authors - Qinghua Huang
View Article