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验证数据展示

  • WB analysis of SW 1990 using 66327-1-Ig (same clone as 66327-1-PBS)

    WB analysis of SW 1990 using 66327-1-Ig (same clone as 66327-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

    IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

    IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

    IF Staining of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

VPS37A Monoclonal antibody, PBS Only

VPS37A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66327-1-PBS

产品说明书

CloneNo. 1F9A4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66327-1-PBS targets VPS37A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19108

Product name: Recombinant human VPS37A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-174 aa of BC022363

Sequence: MPDVPDAFPELSELSVSQLTDMNEQEEVLLEQFLTLPQLKQIITDKDDLVKSIEELARKNLLLEPSLEAKRQTVLDKYELLTQMKSTFEKKMQRQHELSESCSASALQARLKVAAHEAEEESDNIAEDFLEGKMEIDDFLSSFMEKRTICHCRRAKEEKLQQAIAMHSQFHAPL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 37 homolog A (S. cerevisiae)
别名 ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC022363
基因名称 VPS37A
Gene ID (NCBI) 137492
RRIDAB_2881708
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NEZ2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

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