验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68197 targets VPS25 in IF/ICC applications and shows reactivity with Human, Rabbit samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Rabbit |
| 免疫原 |
CatNo: Ag8243 Product name: Recombinant human VPS25 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-176 aa of BC006282 Sequence: MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 25 homolog (S. cerevisiae) |
| 别名 | DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25 |
| 计算分子量 | 176 aa, 21 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC006282 |
| 基因名称 | VPS25 |
| Gene ID (NCBI) | 84313 |
| RRID | AB_3084438 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9BRG1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VPS25 antibody CL488-68197 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
