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验证数据展示

  • WB analysis using 68197-1-Ig

    WB analysis using 68197-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68197-1-Ig

    IF Staining of HepG2 using 68197-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of rabbit kidney using 68197-1-Ig

    WB analysis of rabbit kidney using 68197-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

VPS25 Monoclonal antibody

VPS25 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68197-1-Ig

产品说明书

CloneNo. 3B4C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rabbit

应用

WB, IF/ICC, ELISA

别名

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, rabbit kidney tissue, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68197-1-Ig targets VPS25 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Rabbit
免疫原

CatNo: Ag8243

Product name: Recombinant human VPS25 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-176 aa of BC006282

Sequence: MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 25 homolog (S. cerevisiae)
别名 DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25
计算分子量 176 aa, 21 kDa
观测分子量19 kDa
GenBank蛋白编号BC006282
基因名称 VPS25
Gene ID (NCBI) 84313
RRIDAB_2935286
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRG1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

实验方案

Product Specific Protocols
IF protocol for VPS25 antibody 68197-1-IgDownload protocol
WB protocol for VPS25 antibody 68197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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