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验证数据展示

  • WB analysis using 68197-1-Ig (same clone as 68197-1-PBS)

    WB analysis using 68197-1-Ig (same clone as 68197-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68197-1-Ig (same clone as 68197-1-PBS)

    IF Staining of HepG2 using 68197-1-Ig (same clone as 68197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51765-1

    Cytometric bead array sample test of MP51765-1

    Cytometric bead array sample test of MP51765-1, VPS25 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68197-1-PBS. Detection antibody: 68197-2-PBS.

  • WB analysis of rabbit kidney using 68197-1-Ig (same clone as 68197-1-PBS)

    WB analysis of rabbit kidney using 68197-1-Ig (same clone as 68197-1-PBS)

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51765-1

    Cytometric bead array standard curve of MP51765-1

    Cytometric bead array standard curve of MP51765-1, VPS25 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68197-1-PBS. Detection antibody: 68197-2-PBS. Standard:Ag8243. Range: 0.156-20 ng/mL.

VPS25 Monoclonal antibody, PBS Only (Capture)

VPS25 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68197-1-PBS

产品说明书

CloneNo. 3B4C3

宿主/亚型

Mouse / IgG1

种属反应性

human, rabbit

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

3B4C3, Dermal papilla-derived protein 9, DERP9, EAP20, ELL-associated protein of 20 kDa

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.

产品信息

68197-1-PBS targets VPS25 as part of a matched antibody pair:

MP51765-1: 68197-1-PBS capture and 68197-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rabbit
免疫原

CatNo: Ag8243

Product name: Recombinant human VPS25 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-176 aa of BC006282

Sequence: MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vacuolar protein sorting 25 homolog (S. cerevisiae)
别名 3B4C3, Dermal papilla-derived protein 9, DERP9, EAP20, ELL-associated protein of 20 kDa
计算分子量 176 aa, 21 kDa
观测分子量19 kDa
GenBank蛋白编号BC006282
基因名称 VPS25
Gene ID (NCBI) 84313
RRIDAB_2935286
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRG1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

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