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验证数据展示

  • IF Staining of HepG2 using CL488-66176

    IF Staining of HepG2 using CL488-66176

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated USP13 antibody (CL488-66176, Clone: 6E8C10 ) at dilution of 1:100.

  • IF Staining of HepG2 using CL488-66176

    IF Staining of HepG2 using CL488-66176

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 USP13 antibody (CL488-66176, Clone: 6E8C10 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated USP13 Monoclonal antibody

USP13 Monoclonal Antibody for IF/ICC
Cat No. CL488-66176

产品说明书

CloneNo. 6E8C10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

Ubiquitin carboxyl-terminal hydrolase 13, ISOT-3, ISOT3, Isopeptidase T-3, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66176-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66176 targets USP13 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag10506

Product name: Recombinant human USP13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 515-863 aa of BC016146

Sequence: IAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 13 (isopeptidase T-3)
别名 Ubiquitin carboxyl-terminal hydrolase 13, ISOT-3, ISOT3, Isopeptidase T-3, EC:3.4.19.12
计算分子量 863 aa, 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC016146
基因名称 USP13
Gene ID (NCBI) 8975
RRIDAB_2934450
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92995
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP13 antibody CL488-66176Download protocol
Standard Protocols
Click here to view our Standard Protocols