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验证数据展示

  • WB analysis of HeLa using 66176-1-Ig

    WB analysis of HeLa using 66176-1-Ig

    WB result of USP13 antibody (66176-1-Ig, 1:600) with si-Control and si-USP13 transfected HeLa cells.

  • WB analysis using 66176-1-Ig

    WB analysis using 66176-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human testis using 66176-1-Ig

    IHC staining of human testis using 66176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 66176-1-Ig

    IHC staining of human testis using 66176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 66176-1-Ig

    IF Staining of HepG2 using 66176-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66176-1-Ig (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • IF Staining of HepG2 using 66176-1-Ig

    IF Staining of HepG2 using 66176-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP13 antibody (66176-1-Ig, Clone: 6E8C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HepG2 using 66176-1-Ig

    WB analysis of HepG2 using 66176-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

USP13 Monoclonal antibody

USP13 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66176-1-Ig

产品说明书

CloneNo. 6E8C10
引用文献(3)

宿主/亚型

Mouse / IgG1

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

6E8C10, EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, HepG2 cells, A375 cells, HEK-293 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman testis tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66176-1-Ig targets USP13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag10506

Product name: Recombinant human USP13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 515-863 aa of BC016146

Sequence: IAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 13 (isopeptidase T-3)
别名 6E8C10, EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3
计算分子量 863 aa, 97 kDa
观测分子量97-100 kDa
GenBank蛋白编号BC016146
基因名称 USP13
Gene ID (NCBI) 8975
RRIDAB_2881571
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92995
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for USP13 antibody 66176-1-IgDownload protocol
IHC protocol for USP13 antibody 66176-1-IgDownload protocol
WB protocol for USP13 antibody 66176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Front Mol Biosci

Deubiquitination of MITF-M Regulates Melanocytes Proliferation and Apoptosis.

Authors - Shuaishuai Hu
  • KD Validated
View Article
mouse,humanWB

Biochim Biophys Acta Mol Basis Dis

USP13 inhibition exacerbates mitochondrial dysfunction and acute kidney injury by acting on MCL-1

Authors - Qian Wang
View Article
WB

Sci Transl Med

USP5 inhibition enables potential therapy for t(8;21) AML through ubiquitin-mediated AML1-ETO degradation in patient-derived xenografts

Authors - Lan Ma
View Article
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