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UQCRFS1 Monoclonal antibody

UQCRFS1 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F11C4

Cat No : 68529-1-Ig

Print datasheet

Synonyms

Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1

验证数据展示

  • WB analysis using 68529-1-Ig

    WB analysis using 68529-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68529-1-Ig (UQCRFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68529-1-Ig

    IF Staining of HeLa using 68529-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UQCRFS1 antibody (68529-1-Ig, Clone: 1F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UQCRFS1 Ready-To-Use IHC Kit. UQCRFS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68529-1-Ig targets UQCRFS1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen UQCRFS1 fusion protein Ag13307 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
Synonyms Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC010035
Gene Symbol UQCRFS1
Gene ID (NCBI) 7386
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP47985
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.

实验方案

Product Specific Protocols
WB protocol for UQCRFS1 antibody 68529-1-IgDownload protocol
IF protocol for UQCRFS1 antibody 68529-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols