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验证数据展示

  • WB analysis using 68529-1-Ig (same clone as 68529-1-PBS)

    WB analysis using 68529-1-Ig (same clone as 68529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68529-1-Ig (UQCRFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68529-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68529-1-Ig (same clone as 68529-1-PBS)

    IF Staining of HeLa using 68529-1-Ig (same clone as 68529-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UQCRFS1 antibody (68529-1-Ig, Clone: 1F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68529-1-PBS in a different storage buffer formulation.

UQCRFS1 Monoclonal antibody, PBS Only

UQCRFS1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68529-1-PBS

产品说明书

CloneNo. 1F11C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

Cytochrome b-c1 complex subunit Rieske, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 5, Complex III subunit IX, Complex III subunit 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy UQCRFS1 Ready-To-Use IHC Kit. UQCRFS1 primary antibody included.

产品信息

68529-1-PBS targets UQCRFS1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag13307

Product name: Recombinant human UQCRFS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-274 aa of BC010035

Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
别名 Cytochrome b-c1 complex subunit Rieske, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 5, Complex III subunit IX, Complex III subunit 5
计算分子量 30 kDa
观测分子量 25 kDa
GenBank蛋白编号BC010035
基因名称 UQCRFS1
Gene ID (NCBI) 7386
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP47985
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.

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