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CoraLite® Plus 488-conjugated UBE2C Monoclonal antibody

UBE2C Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C12H8

Cat No : CL488-66087

Print datasheet

Synonyms

dJ447F3.2, UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

验证数据展示

  • IF Staining of HeLa using CL488-66087

    IF Staining of HeLa using CL488-66087

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 UBE2C antibody (CL488-66087, Clone: 1C12H8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66087

    FC experiment of HeLa using CL488-66087

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UBE2C (CL488-66087, Clone:1C12H8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UBE2C Ready-To-Use IHC Kit. UBE2C primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66087-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66087 targets UBE2C in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen UBE2C fusion protein Ag19235 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2C
Synonyms dJ447F3.2, UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC016292
Gene Symbol UBE2C
Gene ID (NCBI) 11065
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00762
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UBE2C antibody CL488-66087Download protocol
FC protocol for CL Plus 488 UBE2C antibody CL488-66087Download protocol
Standard Protocols
Click here to view our Standard Protocols