验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive Dot Blot detected in | / / |
| Positive ChIP-qPCR detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86992-1-RR targets Tri-Methyl-Histone H3 (Lys27) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | H3, H3 Me Lys27, H3 MeK27, H3 Methyl K27, H3 Methyl Lys27 |
| 观测分子量 | 15-17 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
H3K27me3 is a typical transcriptional repression marker that primarily silences key developmental genes through synergistic action with polycomb group proteins. In embryonic stem cells, many developmental regulatory genes are marked by H3K27me3 and remain silent; during cell differentiation, the H3K27me3 markers for specific genes are removed, thereby activating the corresponding developmental programs.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Tri-Methyl-Histone H3 (Lys27) antibody 86992-1-RR | Download protocol |
| WB protocol for Tri-Methyl-Histone H3 (Lys27) antibody 86992-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Signal Role of EZH2 in regulating c-Myc stability through the E3 ubiquitin ligase HUWE1 in heart failure and its mechanisms. | ||
J Inflamm Res The EZH2 Inhibitor GSK126 Alleviates Thromboinflammation in Deep Vein Thrombosis by Suppressing TLR4 Signaling via H3K27me3 Modulation. | ||
Arch Pharm Res Title: Resveratrol ameliorates liver fibrosis by inhibiting ATF4 to regulate glutamine metabolism in hepatic stellate cells. |
