Dot blot analysis was used to confirm the specificity of 86992-1-RR Tri-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Tri-Methyl-Histone H3 (Lys27) (86992-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 86992-1-RR (Tri-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Tri-Methyl-Histone H3 (Lys27) antibody (86992-1-RR, Clone: 252044D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.