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CoraLite®594-conjugated Transketolase Monoclonal antibody

Transketolase Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E2F3

Cat No : CL594-66016

Print datasheet

Synonyms

FLJ34765, TK, TKT, TKT1, transketolase

验证数据展示

  • IF Staining of HepG2 using CL594-66016

    IF Staining of HepG2 using CL594-66016

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated Transketolase antibody (CL594-66016, Clone: 3E2F3 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TKT Ready-To-Use IHC Kit. TKT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66016-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66016 is Transketolase Fusion Protein expressed in E. coli.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen Transketolase fusion protein Ag16779 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transketolase
Synonyms FLJ34765, TK, TKT, TKT1, transketolase
Calculated Molecular Weight 68 kDa
GenBank Accession NumberBC009970
Gene Symbol TKT
Gene ID (NCBI) 7086
RRIDAB_2883478
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29401
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

实验方案

Product Specific Protocols
IF protocol for CL594 Transketolase antibody CL594-66016Download protocol
Standard Protocols
Click here to view our Standard Protocols