Transketolase Monoclonal antibody
Transketolase Monoclonal Antibody for FC, IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
FC, IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3E2F3
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human liver tissue, Jurkat cells, human brain tissue, HEK-293 cells |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TKT Ready-To-Use IHC Kit. TKT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66016-1-Ig targets Transketolase in WB, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IF/ICC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Transketolase fusion protein Ag16779 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transketolase |
| Synonyms | TK, TKT, TKT1, Transketolase |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC009970 |
| Gene Symbol | TKT |
| Gene ID (NCBI) | 7086 |
| RRID | AB_11064729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P29401 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Transketolase antibody 66016-1-Ig | Download protocol |
| IHC protocol for Transketolase antibody 66016-1-Ig | Download protocol |
| IF protocol for Transketolase antibody 66016-1-Ig | Download protocol |
| FC protocol for Transketolase antibody 66016-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Med Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma |
