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验证数据展示

  • FC experiment of RAW 264.7 using 98114-1-RR (same clone as 98114-1-PBS)

    FC experiment of RAW 264.7 using 98114-1-RR (same clone as 98114-1-PBS)

    1x10^6 untreated or LPS-treated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit RecAb (98114-1-RR, Clone: 240875G1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98114-1-PBS in a different storage buffer formulation.

  • FC experiment of RAW 264.7 using 98114-1-RR (same clone as 98114-1-PBS)

    FC experiment of RAW 264.7 using 98114-1-RR (same clone as 98114-1-PBS)

    1x10^6 LPS-treated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit RecAb (98114-1-RR, Clone: 240875G1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Untreated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit RecAb (98114-1-RR, Clone: 240875G1) and PE-conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98114-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98114-1-RR

    Affinity and Kinetic Characterization of 98114-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98114-1-RR against Mouse Tissue Factor/CD142 were performed. The affinity constant is 0.442 nM.

Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody, PBS Only

Tissue Factor/CD142 Uni-rAb® Recombinant Antibody for FC
Cat No. 98114-1-PBS

产品说明书

CloneNo. 240875G1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD142, Coagulation factor III, F3, Tissue factor, 240875G1

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98114-1-PBS targets Tissue Factor/CD142 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1102

Product name: Recombinant Mouse Tissue Factor protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 29-251 aa of

Sequence: AGIPEKAFNLTWISTDFKTILEWQPKPTNYTYTVQISDRSRNWKNKCFSTTDTECDLTDEIVKDVTWAYEAKVLSVPRRNSVHGDGDQLVIHGEEPPFTNAPKFLPYRDTNLGQPVIQQFEQDGRKLNVVVKDSLTLVRKNGTFLTLRQVFGKDLGYIITYRKGSSTGKKTNITNTNEFSIDVEEGVSYCFFVQAMIFSRKTNQNSPGSSTVCTEQWKSFLGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coagulation factor III
别名 CD142, Coagulation factor III, F3, Tissue factor, 240875G1
计算分子量33 kDa
基因名称 Tissue Factor
Gene ID (NCBI) 14066
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP20352
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named CD142, coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

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