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验证数据展示

  • FC experiment of RAW 264.7 using 98114-1-RR

    FC experiment of RAW 264.7 using 98114-1-RR

    1x10^6 untreated or LPS-treated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody (98114-1-RR, Clone: 240875G1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of RAW 264.7 using 98114-1-RR

    FC experiment of RAW 264.7 using 98114-1-RR

    1x10^6 LPS-treated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody (98114-1-RR, Clone: 240875G1) (red) or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody (98114-1-RR, Clone: 240875G1) (black, dashed) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98114-1-RR

    Affinity and Kinetic Characterization of 98114-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98114-1-RR against Mouse Tissue Factor/CD142 were performed. The affinity constant is 0.442 nM.

Anti-Mouse Tissue Factor/CD142 Rabbit Recombinant Antibody

Tissue Factor/CD142 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98114-1-RR

产品说明书

CloneNo. 240875G1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

F3, Coagulation factor III, CD142, Tissue factor, TFA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inLPS treated RAW 264.7 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98114-1-RR targets Tissue Factor/CD142 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coagulation factor III
别名 F3, Coagulation factor III, CD142, Tissue factor, TFA
计算分子量33kDa
基因名称 Tissue Factor
Gene ID (NCBI) 14066
RRIDAB_3672261
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP20352
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named CD142, coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

实验方案

Product Specific Protocols
FC protocol for Tissue Factor/CD142 antibody 98114-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols