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验证数据展示

  • WB analysis using 82540-1-RR

    WB analysis using 82540-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82540-1-RR (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82540-1-RR

    IP experiment of HeLa using 82540-1-RR

    IP result of anti-Tim23 (IP:82540-1-RR, 4ug; Detection:82540-1-RR 1:1000) with HeLa cells lysate 1280 ug.

  • IHC staining of mouse heart using 82540-1-RR

    IHC staining of mouse heart using 82540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82540-1-RR (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 82540-1-RR

    IF Staining of HepG2 using 82540-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (82540-1-RR, Clone: 1H16 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82540-1-RR

    Affinity and Kinetic Characterization of 82540-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82540-1-RR against Human Tim23 were performed. The affinity constant is below 1 pM.

Tim23 Recombinant antibody

Tim23 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 82540-1-RR

产品说明书

CloneNo. 1H16

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TIMM23, TIMM 23, Mitochondrial import inner membrane translocase subunit Tim23, 1H16

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82540-1-RR targets Tim23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Tim23 fusion protein Ag1620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 23
别名 TIMM23, TIMM 23, Mitochondrial import inner membrane translocase subunit Tim23, 1H16
计算分子量 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC062707
基因名称 TIMM23
Gene ID (NCBI) 100287932
RRIDAB_3086488
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14925
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor. Tim23 is the main component of the translocation channel of the TIM23 complex. (PMID: 10830167, PMID: 25083920)

实验方案

Product Specific Protocols
WB protocol for Tim23 antibody 82540-1-RRDownload protocol
IHC protocol for Tim23 antibody 82540-1-RRDownload protocol
IF protocol for Tim23 antibody 82540-1-RRDownload protocol
IP protocol for Tim23 antibody 82540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols