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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82540-1-RR (same clone as 82540-1-PBS)

    WB analysis using 82540-1-RR (same clone as 82540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82540-1-RR (TIM23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82540-1-RR (same clone as 82540-1-PBS)

    IP experiment of HeLa using 82540-1-RR (same clone as 82540-1-PBS)

    IP result of anti-TIM23 (IP:82540-1-RR, 4ug; Detection:82540-1-RR 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 82540-1-RR (same clone as 82540-1-PBS)

    IHC staining of mouse heart using 82540-1-RR (same clone as 82540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82540-1-RR (TIM23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82540-1-RR (same clone as 82540-1-PBS)

    IF Staining of HepG2 using 82540-1-RR (same clone as 82540-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIM23 antibody (82540-1-RR, Clone: 1H16 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82540-1-RR

    Affinity and Kinetic Characterization of 82540-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82540-1-RR against Human Tim23 were performed. The affinity constant is below 1 pM.

TIM23 Recombinant antibody, PBS Only

TIM23 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82540-1-PBS

产品说明书

CloneNo. 1H16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

TIMM23, 1H16, Mitochondrial import inner membrane translocase subunit Tim23, TIMM 23

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82540-1-PBS targets TIM23 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TIM23 fusion protein Ag1620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 23
别名 TIMM23, 1H16, Mitochondrial import inner membrane translocase subunit Tim23, TIMM 23
计算分子量 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC062707
基因名称 TIMM23
Gene ID (NCBI) 100287932
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14925
储存缓冲液 PBS only{{ptg:BufferTemp}}7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor. Tim23 is the main component of the translocation channel of the TIM23 complex. (PMID: 10830167, PMID: 25083920)