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验证数据展示

  • WB analysis using 80128-3-RR (same clone as 80128-3-PBS)

    WB analysis using 80128-3-RR (same clone as 80128-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80128-3-RR (TUBB3-specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

  • Sample test of MP00550-2

    Sample test of MP00550-2

    The mean TUBB3 concentration was determined to be 6.4 ng/mL in SH-SY5Y cell extract based on a 1.4 mg/mL extract load.

  • IF Staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

    IF Staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using TUBB3-specific antibody (80128-3-RR, Clone: 250851G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00550-2

    Cytometric bead array standard curve of MP00550-2

    Cytometric bead array standard curve of MP00550-2, TUBB3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80128-3-PBS. Detection antibody: 80128-2-PBS. Standard: SY01676. Range: 0.039-2.5 ng/mL.

TUBB3-specific/TUJ1 Recombinant antibody, PBS Only (Capture)

TUBB3-specific/TUJ1 Uni-rAbTM Recombinant Antibody for WB, IF-P, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 80128-3-PBS

产品说明书

CloneNo. 250851G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, Cytometric bead array, Indirect ELISA, Sample test

别名

beta tubulin, Tubulin beta 3 chain, Tubulin beta III, TUBB4, Tubulin beta-3 chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

产品信息

80128-3-PBS targets TUBB3-specific/TUJ1 as part of a matched antibody pair:

MP00550-2: 80128-3-PBS capture and 80128-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-P, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tubulin, beta 3
别名 beta tubulin, Tubulin beta 3 chain, Tubulin beta III, TUBB4, Tubulin beta-3 chain
计算分子量 55 kDa
观测分子量 50-55 kDa
GenBank蛋白编号NM_001197181
基因名称 TUBB3
Gene ID (NCBI) 10381
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13509
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C