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验证数据展示

  • WB analysis using 80128-3-RR

    WB analysis using 80128-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80128-3-RR (TUBB3-specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 80128-3-RR

    IHC staining of mouse cerebellum using 80128-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of mouse cerebellum using 80128-3-RR

    IHC staining of mouse cerebellum using 80128-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IF Staining of mouse cerebellum using 80128-3-RR

    IF Staining of mouse cerebellum using 80128-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using TUBB3-specific antibody (80128-3-RR, Clone: 250851G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TUBB3-specific/TUJ1 Recombinant antibody

TUBB3-specific/TUJ1 Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 80128-3-RR

产品说明书

CloneNo. 250851G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

beta tubulin, Tubulin beta 3 chain, Tubulin beta III, TUBB4, Tubulin beta-3 chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells, SH-SY5Y cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80128-3-RR targets TUBB3-specific/TUJ1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tubulin, beta 3
别名 beta tubulin, Tubulin beta 3 chain, Tubulin beta III, TUBB4, Tubulin beta-3 chain
计算分子量 55 kDa
观测分子量 50-55 kDa
GenBank蛋白编号NM_001197181
基因名称 TUBB3
Gene ID (NCBI) 10381
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13509
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TUBB3-specific/TUJ1 antibody 80128-3-RRDownload protocol
IHC protocol for TUBB3-specific/TUJ1 antibody 80128-3-RRDownload protocol
IF protocol for TUBB3-specific/TUJ1 antibody 80128-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols