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验证数据展示

  • WB analysis using 25701-1-AP (same clone as 25701-1-PBS)

    WB analysis using 25701-1-AP (same clone as 25701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 25701-1-AP (same clone as 25701-1-PBS)

    WB analysis of HeLa using 25701-1-AP (same clone as 25701-1-PBS)

    WB result of TTC28 antibody (25701-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC28 transfected HeLa cells. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 25701-1-AP (same clone as 25701-1-PBS)

    IP experiment of HEK-293 using 25701-1-AP (same clone as 25701-1-PBS)

    IP result of anti-TTC28 (IP:25701-1-AP, 4ug; Detection:25701-1-AP 1:2500) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TTC28 Polyclonal antibody, PBS Only

TTC28 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25701-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio, TPRBK

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TTC28 Ready-To-Use IHC Kit. TTC28 primary antibody included.

产品信息

25701-1-PBS targets TTC28 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22465

Product name: Recombinant human TTC28 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 906-1247 aa of BC016465

Sequence: TGESDQSSTETDSTVKSQEESNPKLDPQELAQKILEETQSHLIAVERLQRSGGQVSKSNNPEDGVQAPSSTAVFRASETSAFSRPVLSHQKSQPSPVTVKPKPPARSSSLPKVSSGYSSPTTSEMSIKDSPSQHSGRPSPGCDSQTSQLDQPLFKLKYPSSPYSAHISKSPRNMSPSSGHQSPAGSAPSPALSYSSAGSARSSPADAPDIDKLKMAAIDEKVQAVHNLKMFWQSTPQHSTGPMKIFRGAPGTMTSKRDVLSLLNLSPRHNKKEEGVDKLELKELSLQQHDGAPPKAPPNGHWRTETTSLGSLPLPAGPPATAPARPLRLPSGNGYKFLSPGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 28
别名 TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio, TPRBK
计算分子量 2481 aa, 271 kDa
观测分子量240-271 kDa
GenBank蛋白编号BC016465
基因名称 TTC28
Gene ID (NCBI) 23331
RRIDAB_3085817
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96AY4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression.

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