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验证数据展示

  • WB analysis using 25701-1-AP

    WB analysis using 25701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25701-1-AP

    WB analysis of HeLa using 25701-1-AP

    WB result of TTC28 antibody (25701-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC28 transfected HeLa cells.

  • IP experiment of HEK-293 using 25701-1-AP

    IP experiment of HEK-293 using 25701-1-AP

    IP result of anti-TTC28 (IP:25701-1-AP, 4ug; Detection:25701-1-AP 1:2500) with HEK-293 cells lysate 1480 ug.

  • Featured Product
  • KD/KO Validated

TTC28 Polyclonal antibody

TTC28 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25701-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio, TPRBK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, LNCaP cells
Positive IP detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TTC28 Ready-To-Use IHC Kit. TTC28 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25701-1-AP targets TTC28 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22465

Product name: Recombinant human TTC28 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 906-1247 aa of BC016465

Sequence: TGESDQSSTETDSTVKSQEESNPKLDPQELAQKILEETQSHLIAVERLQRSGGQVSKSNNPEDGVQAPSSTAVFRASETSAFSRPVLSHQKSQPSPVTVKPKPPARSSSLPKVSSGYSSPTTSEMSIKDSPSQHSGRPSPGCDSQTSQLDQPLFKLKYPSSPYSAHISKSPRNMSPSSGHQSPAGSAPSPALSYSSAGSARSSPADAPDIDKLKMAAIDEKVQAVHNLKMFWQSTPQHSTGPMKIFRGAPGTMTSKRDVLSLLNLSPRHNKKEEGVDKLELKELSLQQHDGAPPKAPPNGHWRTETTSLGSLPLPAGPPATAPARPLRLPSGNGYKFLSPGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 28
别名 TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio, TPRBK
计算分子量 2481 aa, 271 kDa
观测分子量240-271 kDa
GenBank蛋白编号BC016465
基因名称 TTC28
Gene ID (NCBI) 23331
RRIDAB_3085817
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96AY4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression.

实验方案

Product Specific Protocols
IP protocol for TTC28 antibody 25701-1-APDownload protocol
WB protocol for TTC28 antibody 25701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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