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验证数据展示

  • IF Staining of HeLa using CL594-67381

    IF Staining of HeLa using CL594-67381

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 TSG101 antibody (CL594-67381, Clone: 2B7G8 ) at dilution of 1:200.

CoraLite®594-conjugated TSG101 Monoclonal antibody

TSG101 Monoclonal Antibody for IF/ICC
Cat No. CL594-67381

产品说明书

CloneNo. 2B7G8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

IF/ICC

别名

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67381-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67381 targets TSG101 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag28569

Product name: Recombinant human TSG101 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 214-390 aa of BC002487

Sequence: GPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTILYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor susceptibility gene 101
别名 ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23
计算分子量 44 kDa
观测分子量 43-46 kDa
GenBank蛋白编号BC002487
基因名称 TSG101
Gene ID (NCBI) 7251
RRIDAB_3084763
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99816
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 TSG101 antibody CL594-67381Download protocol
Standard Protocols
Click here to view our Standard Protocols