验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue |
| Positive FC (Intra) detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 1 publications below |
产品信息
CL488-67381 targets TSG101 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 文献引用应用 | FC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | goat |
| 免疫原 |
CatNo: Ag28569 Product name: Recombinant human TSG101 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 214-390 aa of BC002487 Sequence: GPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTILYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tumor susceptibility gene 101 |
| 别名 | VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 43-46 kDa |
| GenBank蛋白编号 | BC002487 |
| 基因名称 | TSG101 |
| Gene ID (NCBI) | 7251 |
| RRID | AB_2919462 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99816 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TSG101 antibody CL488-67381 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |