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验证数据展示

  • WB analysis of mouse brain using 18236-1-AP (same clone as 18236-1-PBS)

    WB analysis of mouse brain using 18236-1-AP (same clone as 18236-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • WB analysis using 18236-1-AP (same clone as 18236-1-PBS)

    WB analysis using 18236-1-AP (same clone as 18236-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

    IHC staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

    IHC staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 18236-1-AP (same clone as 18236-1-PBS)

    IP experiment of mouse lung using 18236-1-AP (same clone as 18236-1-PBS)

    IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 18236-1-AP (same clone as 18236-1-PBS)

    FC experiment of A549 using 18236-1-AP (same clone as 18236-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

    IF Staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using TRPC6 antibody (18236-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 18236-1-AP (same clone as 18236-1-PBS)

    IF Staining of A549 using 18236-1-AP (same clone as 18236-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRPC6 Polyclonal antibody, PBS Only

TRPC6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 18236-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

别名

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRP-6

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18236-1-PBS targets TRPC6 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12976

Product name: Recombinant human TRPC6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-405 aa of BC093658

Sequence: MSQSPAFGPRRGSSPRGAAGAAARRNESQDYLLMDSELGEDGCPQAPLPCYGYYPCFRGSDNRLAHRRQTVLREKGRRLANRGPAYMFSDRSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAVANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILSHPAFAEGKRLATSPSQSELQQDDFYAYDEDGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCNDCNQKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYKKLSMQCKDFVVGLLDLCRNTEEVEAILNGDVETLQSGDHGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily C, member 6
别名 FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRP-6
计算分子量 931 aa, 106 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC093658
基因名称 TRPC6
Gene ID (NCBI) 7225
RRIDAB_10859822
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y210
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

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