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验证数据展示

  • IF Staining of HeLa using CL488-14208

    IF Staining of HeLa using CL488-14208

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TRIM24 antibody (CL488-14208) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-14208

    FC experiment of HeLa using CL488-14208

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated TRIM24 Polyclonal antibody (CL488-14208)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TRIM24 Polyclonal antibody

TRIM24 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14208

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

TRIM24, tripartite motif containing 24, Tripartite motif containing protein 24, Tripartite motif-containing protein 24, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1, TIF1A, TIF1-alpha, Transcription intermediary factor 1-alpha, TRIM, TRIM 24

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIM24 Ready-To-Use IHC Kit. TRIM24 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14208-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14208 targets TRIM24 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5417

Product name: Recombinant human TRIM24 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 701-1050 aa of BC028689

Sequence: SKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 24
别名 TRIM24, tripartite motif containing 24, Tripartite motif containing protein 24, Tripartite motif-containing protein 24, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1, TIF1A, TIF1-alpha, Transcription intermediary factor 1-alpha, TRIM, TRIM 24
计算分子量 117 kDa
观测分子量 117 kDa
GenBank蛋白编号BC028689
基因名称 TRIM24
Gene ID (NCBI) 8805
RRIDAB_3672584
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15164
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TRIM24 antibody CL488-14208Download protocol
IF protocol for CL Plus 488 TRIM24 antibody CL488-14208Download protocol
Standard Protocols
Click here to view our Standard Protocols
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