验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, BGC-823 cells, human heart tissue, mouse skeletal muscle tissue, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TRIM24 Ready-To-Use IHC Kit. TRIM24 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14208-1-AP targets TRIM24 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TRIM24 fusion protein Ag5417 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 24 |
| 别名 | TIF1-alpha, TIF1A, TIF1, RING-type E3 ubiquitin transferase TIF1-alpha, EC:2.3.2.27 |
| 计算分子量 | 117 kDa |
| 观测分子量 | 117 kDa |
| GenBank蛋白编号 | BC028689 |
| 基因名称 | TRIM24 |
| Gene ID (NCBI) | 8805 |
| RRID | AB_2256646 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15164 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM24 antibody 14208-1-AP | Download protocol |
| IHC protocol for TRIM24 antibody 14208-1-AP | Download protocol |
| IF protocol for TRIM24 antibody 14208-1-AP | Download protocol |
| IP protocol for TRIM24 antibody 14208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression
| ||
Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains. | ||
Nat Chem Biol Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands. | ||
