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验证数据展示

  • WB analysis using 24555-1-AP (same clone as 24555-1-PBS)

    WB analysis using 24555-1-AP (same clone as 24555-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

    WB analysis of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

    WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

    IP experiment of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

    IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRAPPC12 Polyclonal antibody, PBS Only

TRAPPC12 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 24555-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24555-1-PBS targets TRAPPC12 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18332

Product name: Recombinant human TTC15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 395-735 aa of BC014164

Sequence: AAVDLCGRLLTAHGQGYGKSGLLTSHTTDSLQLWFVRLALLVKLGLFQNAEMEFEPFGNLDQPDLYYEYYPHVYPGRRGSMVPFSMRILHAELQQYLGNPQESLDRLHKVKTVCSKILANLEQGLAEDGGMSSVTQEGRQASIRLWRSRLGRVMYSMANCLLLMKDYVLAVDAYHSVIKYYPEQEPQLLSGIGRISLQIGDIKTAEKYFQDVEKVTQKLDGLQGKIMVLMNSAFLHLGQNNFAEAHRFFTEILRMDPRNAVANNNAAVCLLYLGKLKDSLRQLEAMVQQDPRHYLHESVLFNLTTMYELESSRSMQKKQALLEAVAGKEGDSFNTQCLKLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 15
别名 TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis
计算分子量 735 aa, 79 kDa
观测分子量98 kDa
GenBank蛋白编号BC014164
基因名称 TRAPPC12
Gene ID (NCBI) 51112
RRIDAB_3742117
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVT3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).

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