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验证数据展示

  • WB analysis using 24555-1-AP

    WB analysis using 24555-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 24555-1-AP

    WB analysis of K-562 using 24555-1-AP

    WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells.

  • IP experiment of K-562 using 24555-1-AP

    IP experiment of K-562 using 24555-1-AP

    IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug.

  • Featured Product
  • KD/KO验证

TRAPPC12 Polyclonal antibody

TRAPPC12 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24555-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24555-1-AP targets TRAPPC12 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 TRAPPC12 fusion protein Ag18332 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 15
别名 TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis
计算分子量 735 aa, 79 kDa
观测分子量98 kDa
GenBank蛋白编号BC014164
基因名称 TRAPPC12
Gene ID (NCBI) 51112
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVT3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).

实验方案

Product Specific Protocols
WB protocol for TRAPPC12 antibody 24555-1-APDownload protocol
IP protocol for TRAPPC12 antibody 24555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols