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验证数据展示

  • WB analysis using 83535-2-RR

    WB analysis using 83535-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83535-2-RR (TRAF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 83535-2-RR

    IHC staining of rat brain using 83535-2-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRAF6 antibody included

TRAF6 Recombinant antibody

TRAF6 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83535-2-RR

产品说明书

CloneNo. 230430H3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

230430H3, E3 ubiquitin-protein ligase TRAF6, EC:2.3.2.27, Interleukin-1 signal transducer, RING-type E3 ubiquitin transferase TRAF6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRAF6 Ready-To-Use IHC Kit. TRAF6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83535-2-RR targets TRAF6 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 TRAF6 fusion protein Ag14858 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TNF receptor-associated factor 6
别名 230430H3, E3 ubiquitin-protein ligase TRAF6, EC:2.3.2.27, Interleukin-1 signal transducer, RING-type E3 ubiquitin transferase TRAF6
计算分子量 522 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC031052
基因名称 TRAF6
Gene ID (NCBI) 7189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4K3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

实验方案

Product Specific Protocols
WB protocol for TRAF6 antibody 83535-2-RRDownload protocol
IHC protocol for TRAF6 antibody 83535-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols