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Host
0
Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 83535-2-RR (same clone as 83535-2-PBS)

    WB analysis using 83535-2-RR (same clone as 83535-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83535-2-RR (TRAF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83535-2-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 83535-2-RR (same clone as 83535-2-PBS)

    IHC staining of rat brain using 83535-2-RR (same clone as 83535-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83535-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRAF6 antibody included

TRAF6 Recombinant antibody, PBS Only

TRAF6 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83535-2-PBS

产品说明书

CloneNo. 230430H3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

230430H3, E3 ubiquitin-protein ligase TRAF6, EC:2.3.2.27, Interleukin-1 signal transducer, RING-type E3 ubiquitin transferase TRAF6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TRAF6 Ready-To-Use IHC Kit. TRAF6 primary antibody included.

产品信息

83535-2-PBS targets TRAF6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 TRAF6 fusion protein Ag14858 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TNF receptor-associated factor 6
别名 230430H3, E3 ubiquitin-protein ligase TRAF6, EC:2.3.2.27, Interleukin-1 signal transducer, RING-type E3 ubiquitin transferase TRAF6
计算分子量 522 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC031052
基因名称 TRAF6
Gene ID (NCBI) 7189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4K3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.