验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-66713 targets TPT1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 |
CatNo: Ag27651 Product name: Recombinant human TPT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-172 aa of BC003352 Sequence: MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tumor protein, translationally-controlled 1 |
| 别名 | FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1 |
| 计算分子量 | 20 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC003352 |
| 基因名称 | TPT1 |
| Gene ID (NCBI) | 7178 |
| RRID | AB_2934677 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P13693 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 TPT1 antibody CL555-66713 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
