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验证数据展示

  • WB analysis using 66713-1-Ig

    WB analysis using 66713-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66713-1-Ig

    WB analysis of MCF-7 using 66713-1-Ig

    WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

  • IHC staining of human colon cancer using 66713-1-Ig

    IHC staining of human colon cancer using 66713-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPT1 antibody included
  • IF Staining of HEK-293 using 66713-1-Ig

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HEK-293 using 66713-1-Ig

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

TPT1 Monoclonal antibody

TPT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66713-1-Ig

产品说明书

CloneNo. 1G6A10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

TCTP, p23, HRF, Histamine-releasing factor, Fortilin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, RAW 264.7 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TPT1 Ready-To-Use IHC Kit. TPT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66713-1-Ig targets TPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27651

Product name: Recombinant human TPT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-172 aa of BC003352

Sequence: MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor protein, translationally-controlled 1
别名 TCTP, p23, HRF, Histamine-releasing factor, Fortilin
计算分子量 20 kDa
观测分子量 22 kDa
GenBank蛋白编号BC003352
基因名称 TPT1
Gene ID (NCBI) 7178
RRIDAB_2882064
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP13693
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

实验方案

Product Specific Protocols
IF protocol for TPT1 antibody 66713-1-IgDownload protocol
IHC protocol for TPT1 antibody 66713-1-IgDownload protocol
WB protocol for TPT1 antibody 66713-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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