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TPT1 Monoclonal antibody

TPT1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G6A10

Cat No : 66713-1-Ig

Print datasheet

Synonyms

FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1

验证数据展示

  • WB analysis using 66713-1-Ig

    WB analysis using 66713-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66713-1-Ig

    WB analysis of MCF-7 using 66713-1-Ig

    WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

  • IHC staining of human colon cancer using 66713-1-Ig

    IHC staining of human colon cancer using 66713-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPT1 antibody included
  • IF Staining of HEK-293 using 66713-1-Ig

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HEK-293 using 66713-1-Ig

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, RAW 264.7 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TPT1 Ready-To-Use IHC Kit. TPT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66713-1-Ig targets TPT1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen TPT1 fusion protein Ag27651 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor protein, translationally-controlled 1
Synonyms FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003352
Gene Symbol TPT1
Gene ID (NCBI) 7178
RRIDAB_2882064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13693
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

实验方案

Product Specific Protocols
WB protocol for TPT1 antibody 66713-1-IgDownload protocol
IHC protocol for TPT1 antibody 66713-1-IgDownload protocol
IF protocol for TPT1 antibody 66713-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols