验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, HepG2 cells, rat liver tissue, rat kidney tissue, RAW 264.7 cells, mouse liver tissue, mouse kidney tissue, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10713-1-AP targets TPI1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, chicken, yeast, drosophila |
| 免疫原 |
CatNo: Ag1175 Product name: Recombinant human TPI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC007086 Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | triosephosphate isomerase 1 |
| 别名 | Triose-phosphate isomerase, TPI, TIM, Methylglyoxal synthase, EC:5.3.1.1 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC007086 |
| 基因名称 | TPI1 |
| Gene ID (NCBI) | 7167 |
| RRID | AB_2207716 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P60174 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TPI1 antibody 10713-1-AP | Download protocol |
| WB protocol for TPI1 antibody 10713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
Brain Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation. |
