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PIG3 Polyclonal antibody

PIG3 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14828-1-AP

Print datasheet

Synonyms

p53 induced gene 3 protein, PIG3, Quinone oxidoreductase PIG3, TP53I3

验证数据展示

  • WB analysis of HeLa using 14828-1-AP

    WB analysis of HeLa using 14828-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 14828-1-AP

    IP experiment of MCF-7 using 14828-1-AP

    IP result of anti-PIG3 (IP:14828-1-AP, 4ug; Detection:14828-1-AP 1:500) with MCF-7 cells lysate 3200ug.

  • IHC staining of human liver cancer using 14828-1-AP

    IHC staining of human liver cancer using 14828-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 14828-1-AP

    IHC staining of human liver cancer using 14828-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 14828-1-AP

    IF Staining of HepG2 using 14828-1-AP

    Immunofluorescent analysis of HepG2 cells using 14828-1-AP (PIG3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, SMMC-7721 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14828-1-AP targets PIG3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen PIG3 fusion protein Ag6618 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor protein p53 inducible protein 3
Synonyms p53 induced gene 3 protein, PIG3, Quinone oxidoreductase PIG3, TP53I3
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC000474
Gene Symbol TP53I3
Gene ID (NCBI) 9540
RRIDAB_2206893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53FA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIG3 antibody 14828-1-APDownload protocol
IHC protocol for PIG3 antibody 14828-1-APDownload protocol
IF protocol for PIG3 antibody 14828-1-APDownload protocol
IP protocol for PIG3 antibody 14828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Biomaterials

Furin-instructed aggregated gold nanoparticles for re-educating tumor associated macrophages and overcoming breast cancer chemoresistance.

Authors - Rou Xie
View Article