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验证数据展示

  • IF Staining of HepG2 using CL488-14828

    IF Staining of HepG2 using CL488-14828

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PIG3 antibody (CL488-14828) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-14828

    FC experiment of HepG2 using CL488-14828

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Pig3 Polyclonal Antibody (CL488-14828)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PIG3 Polyclonal antibody

PIG3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14828

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

TP53I3, Quinone oxidoreductase PIG3, p53-induced gene 3 protein, p53 induced gene 3 protein, NADPH:quinone reductase PIG3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 14828-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14828 targets PIG3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PIG3 fusion protein Ag6618 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor protein p53 inducible protein 3
别名 TP53I3, Quinone oxidoreductase PIG3, p53-induced gene 3 protein, p53 induced gene 3 protein, NADPH:quinone reductase PIG3
计算分子量 35 kDa
观测分子量 34-36 kDa
GenBank蛋白编号BC000474
基因名称 TP53I3
Gene ID (NCBI) 9540
RRIDAB_3672609
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53FA7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PIG3 antibody CL488-14828Download protocol
FC protocol for CL Plus 488 PIG3 antibody CL488-14828Download protocol
Standard Protocols
Click here to view our Standard Protocols