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验证数据展示

  • WB analysis using 68170-1-Ig

    WB analysis using 68170-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68170-1-Ig

    WB analysis using 68170-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HepG2 using 68170-1-Ig

    WB analysis of HepG2 using 68170-1-Ig

    WB result of TOLLIP antibody (68170-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells.

  • IHC staining of mouse colon using 68170-1-Ig

    IHC staining of mouse colon using 68170-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOLLIP antibody included
  • IHC staining of mouse colon using 68170-1-Ig

    IHC staining of mouse colon using 68170-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOLLIP antibody included
  • IHC staining of rat colon using 68170-1-Ig

    IHC staining of rat colon using 68170-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOLLIP antibody included
  • IF Staining of U2OS using 68170-1-Ig

    IF Staining of U2OS using 68170-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TOLLIP antibody (68170-1-Ig, Clone: 1B3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of Jurkat using 68170-1-Ig

    FC experiment of Jurkat using 68170-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TOLLIP (68170-1-Ig, Clone:1B3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

TOLLIP Monoclonal antibody

TOLLIP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 68170-1-Ig

产品说明书

CloneNo. 1B3C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, human peripheral blood platelets, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy TOLLIP Ready-To-Use IHC Kit. TOLLIP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68170-1-Ig targets TOLLIP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TOLLIP fusion protein Ag32731 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 toll interacting protein
别名 Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4
计算分子量 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC012057
基因名称 TOLLIP
Gene ID (NCBI) 54472
RRIDAB_2923686
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0E2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

实验方案

Product Specific Protocols
WB protocol for TOLLIP antibody 68170-1-IgDownload protocol
IHC protocol for TOLLIP antibody 68170-1-IgDownload protocol
IF protocol for TOLLIP antibody 68170-1-IgDownload protocol
FC protocol for TOLLIP antibody 68170-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols