验证数据展示
经过测试的应用
| Positive IF/ICC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy® TNIK Ready-To-Use IHC Kit. TNIK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84231-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84231-4 targets TNIK in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34132 Product name: Recombinant human TNIK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 585-636 aa of BC150256 Sequence: LRPVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTSHRVEMP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | TRAF2 and NCK interacting kinase |
| 别名 | TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1, 241518D9 |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | BC150256 |
| 基因名称 | TNIK |
| Gene ID (NCBI) | 23043 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UKE5 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TNIK antibody CL488-84231-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
