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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84231-4-RR (same clone as 84231-4-PBS)

    WB analysis using 84231-4-RR (same clone as 84231-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84231-4-RR (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 84231-4-RR (same clone as 84231-4-PBS)

    IHC staining of human colon using 84231-4-RR (same clone as 84231-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84231-4-RR (TNIK antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 84231-4-RR (same clone as 84231-4-PBS)

    IHC staining of human colon using 84231-4-RR (same clone as 84231-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84231-4-RR (TNIK antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 84231-4-RR (same clone as 84231-4-PBS)

    IF Staining of K-562 using 84231-4-RR (same clone as 84231-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (84231-4-RR, Clone: 241518D9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84231-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84231-4-RR

    Affinity and Kinetic Characterization of 84231-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84231-4-RR against Human TNIK were performed. The affinity constant is 2.01 nM.

TNIK Recombinant antibody, PBS Only

TNIK Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84231-4-PBS

产品说明书

CloneNo. 241518D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

241518D9, EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84231-4-PBS targets TNIK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 TNIK fusion protein Ag34132 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRAF2 and NCK interacting kinase
别名 241518D9, EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase
观测分子量155~180 kDa
GenBank蛋白编号BC150256
基因名称 TNIK
Gene ID (NCBI) 23043
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UKE5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNIK (Traf2- and Nck-interacting kinase) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension, which had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling (PMID: 24566388). TNIK is also an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462). Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).