验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive FC detected in | Con A treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98112-1-RR targets TNFRSF9/CD137 in IHC, IF-P, FC applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF-P, FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor receptor superfamily, member 9 |
| 别名 | TNFRSF9, 241344F3, 4 1BB, 4 1BB ligand receptor, 4-1BB ligand receptor |
| 计算分子量 | 28 kDa |
| GenBank蛋白编号 | BC006196 |
| 基因名称 | TNFRSF9/CD137 |
| Gene ID (NCBI) | 3604 |
| RRID | AB_3672259 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q07011 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TNFRSF9/CD137 antibody 98112-1-RR | Download protocol |
| IF protocol for TNFRSF9/CD137 antibody 98112-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
