验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, HEK-293T cells, HeLa cells, mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TMEM97 Ready-To-Use IHC Kit. TMEM97 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
26444-1-AP targets TMEM97 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag23917 Product name: Recombinant human TMEM97 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-69 aa of BC045655 Sequence: MTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 97 |
| 别名 | MAC30, Protein MAC30, S2R, Sigma2 receptor, Sigma-2 receptor |
| 观测分子量 | 19-20 kDa |
| GenBank蛋白编号 | BC045655 |
| 基因名称 | TMEM97 |
| Gene ID (NCBI) | 27346 |
| RRID | AB_2880515 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5BJF2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM97 antibody 26444-1-AP | Download protocol |
| WB protocol for TMEM97 antibody 26444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Chem Biol A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. | ||
ACS Chem Neurosci Sigma Receptor Ligands Are Potent Antiprion Compounds that Act Independently of Sigma Receptor Binding
| ||
J Bioenerg Biomembr Meningioma-associated protein 30 accelerates the proliferation and invasion of hepatocellular carcinoma by modulating Wnt/GSK-3β/β-catenin signaling. | ||
Life Sci Alliance The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth | ||
Cell Biosci PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression |
