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验证数据展示

  • WB analysis using 31525-1-AP (same clone as 31525-1-PBS)

    WB analysis using 31525-1-AP (same clone as 31525-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31525-1-AP (TMEM258 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31525-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 31525-1-AP (same clone as 31525-1-PBS)

    IHC staining of mouse small intestine using 31525-1-AP (same clone as 31525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31525-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TMEM258 antibody included
  • IHC staining of mouse kidney using 31525-1-AP (same clone as 31525-1-PBS)

    IHC staining of mouse kidney using 31525-1-AP (same clone as 31525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31525-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TMEM258 antibody included
  • IHC staining of mouse kidney using 31525-1-AP (same clone as 31525-1-PBS)

    IHC staining of mouse kidney using 31525-1-AP (same clone as 31525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31525-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TMEM258 antibody included

TMEM258 Polyclonal antibody, PBS Only

TMEM258 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31525-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

C11orf10

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® TMEM258 Ready-To-Use IHC Kit. TMEM258 primary antibody included.

产品信息

31525-1-PBS targets TMEM258 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35142

Product name: Recombinant human TMEM258 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-54 aa of BC015968

Sequence: MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 10
别名 C11orf10
观测分子量10 and 16 kDa
GenBank蛋白编号BC015968
基因名称 C11orf10
Gene ID (NCBI) 746
RRIDAB_3742445
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP61165
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM258, or transmembrane protein 258, is a component of the oligosaccharyltransferase (OST) complex. Research has shown that TMEM258 is involved in controlling ER stress and intestinal inflammation. In particular, it has been identified as a central regulator of intestinal homeostasis. The loss of TMEM258 leads to unresolved ER stress, which can result in apoptosis (PMID: 27974209). The calculated MW is 9 kDa, The observed MW is multiple bands, which may be caused by Glycosylation modification.

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