• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 31525-1-AP

    WB analysis using 31525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31525-1-AP (TMEM258 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 31525-1-AP

    IHC staining of mouse small intestine using 31525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM258 antibody included
  • IHC staining of mouse kidney using 31525-1-AP

    IHC staining of mouse kidney using 31525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM258 antibody included
  • IHC staining of mouse kidney using 31525-1-AP

    IHC staining of mouse kidney using 31525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM258 antibody included

TMEM258 Polyclonal antibody

TMEM258 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31525-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C11orf10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, PC-3 cells, SH-SY5Y cells, U2OS cells
Positive IHC detected inmouse kidney tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® TMEM258 Ready-To-Use IHC Kit. TMEM258 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31525-1-AP targets TMEM258 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35142

Product name: Recombinant human TMEM258 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-54 aa of BC015968

Sequence: MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 10
别名 C11orf10
观测分子量10 and 16 kDa
GenBank蛋白编号BC015968
基因名称 C11orf10
Gene ID (NCBI) 746
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP61165
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM258, or transmembrane protein 258, is a component of the oligosaccharyltransferase (OST) complex. Research has shown that TMEM258 is involved in controlling ER stress and intestinal inflammation. In particular, it has been identified as a central regulator of intestinal homeostasis. The loss of TMEM258 leads to unresolved ER stress, which can result in apoptosis (PMID: 27974209). The calculated MW is 9 kDa, The observed MW is multiple bands, which may be caused by Glycosylation modification.

实验方案

Product Specific Protocols
IHC protocol for TMEM258 antibody 31525-1-APDownload protocol
WB protocol for TMEM258 antibody 31525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...