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验证数据展示

  • WB analysis using 31525-1-AP

    WB analysis using 31525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31525-1-AP (TMEM258 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 31525-1-AP

    IHC staining of mouse small intestine using 31525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM258 antibody included
  • IHC staining of mouse kidney using 31525-1-AP

    IHC staining of mouse kidney using 31525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM258 antibody included
  • IHC staining of mouse kidney using 31525-1-AP

    IHC staining of mouse kidney using 31525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31525-1-AP (TMEM258 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM258 antibody included

TMEM258 Polyclonal antibody

TMEM258 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31525-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C11orf10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, PC-3 cells, SH-SY5Y cells, U2OS cells
Positive IHC detected inmouse kidney tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® TMEM258 Ready-To-Use IHC Kit. TMEM258 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31525-1-AP targets TMEM258 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35142

Product name: Recombinant human TMEM258 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-54 aa of BC015968

Sequence: MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 10
别名 C11orf10
观测分子量10 and 16 kDa
GenBank蛋白编号BC015968
基因名称 C11orf10
Gene ID (NCBI) 746
RRIDAB_3742445
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP61165
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM258, or transmembrane protein 258, is a component of the oligosaccharyltransferase (OST) complex. Research has shown that TMEM258 is involved in controlling ER stress and intestinal inflammation. In particular, it has been identified as a central regulator of intestinal homeostasis. The loss of TMEM258 leads to unresolved ER stress, which can result in apoptosis (PMID: 27974209). The calculated MW is 9 kDa, The observed MW is multiple bands, which may be caused by Glycosylation modification.

实验方案

Product Specific Protocols
IHC protocol for TMEM258 antibody 31525-1-APDownload protocol
WB protocol for TMEM258 antibody 31525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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