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验证数据展示

  • WB analysis using 31210-1-AP (same clone as 31210-1-PBS)

    WB analysis using 31210-1-AP (same clone as 31210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31210-1-AP (TMEM174 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31210-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293T using 31210-1-AP (same clone as 31210-1-PBS)

    WB analysis of HEK-293T using 31210-1-AP (same clone as 31210-1-PBS)

    WB result of TMEM174 antibody (31210-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM174 transfected HEK-293T cells. This data was developed using the same antibody clone with 31210-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 31210-1-AP (same clone as 31210-1-PBS)

    IP experiment of Jurkat using 31210-1-AP (same clone as 31210-1-PBS)

    IP result of anti-TMEM174 (IP:31210-1-AP, 4ug; Detection:31210-1-AP 1:800) with Jurkat cells lysate 1360 ug. This data was developed using the same antibody clone with 31210-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMEM174 Polyclonal antibody, PBS Only

TMEM174 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 31210-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

transmembrane protein 174

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31210-1-PBS targets TMEM174 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35041

Product name: Recombinant human TMEM174 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-243 aa of BC019346

Sequence: MEQGSGRLEDFPVNVFSVTPYTPSTADIQVSDDDKAGATLLFSGIFLGLVGITFTVMGWIKYQGVSHFEWTQLLGPVLLSVGVTFILIAVCKFKMLSCQLCKESEERVPDSEQTPGGPSFVFTGINQPITFHGATVVQYIPPPYGSPEPMGINTSYLQSVVSPCGLITSGGAAAAMSSPPQYYTIYPQDNSAFVVDEGCLSFTDGGNHRPNPDVDQLEETQLEEEACACFSPPPYEEIYSLPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 174
别名 transmembrane protein 174
观测分子量 55-60 kDa
GenBank蛋白编号BC019346
基因名称 TMEM174
Gene ID (NCBI) 134288
RRIDAB_3742409
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8WUU8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transmembrane protein 174 (TMEM174) is comprised of 243 amino acids, and contains two predicted transmembrane helices which determine its subcellular localization in endoplasmic reticulum and influences its functions (PMID: 20331980). TMEM174) mRNA is easily detectable in human kidney tissues and activates AP-1 and promotes 293T cell proliferation (PMID: 25202393). The Tmem174 protein was extremely highly expressed in the kidney and localized at the apical membrane of the renal proximal tubules. Tmem174 binds to NaPi2a on the cell membrane and is involved in the internalization of NaPi2a (PMID: 35428804). Tmem174 is considered to be a component of the NaPi2a/NHERF1 complex that receives signals from PTH and FGF23 (PMID: 35428804). This antibody may recognize the dimer.

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